117 research outputs found

    Google, Facebook and what else? Measuring the hybridity of Italian journalists by their use of sources

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    The contemporary media systems present hybrid logics and features that imply an increasingly interdependence among actors, media and communication formats. The hybrid media system approach underlines that legacy news media and non-elite media actors construct flows of news through different media technologies and according to complex temporal structures. A media environment arises in which traditional distinctions between concepts like \u2018online\u2019 and \u2018offline\u2019, \u2018producer\u2019 and \u2018audience\u2019, \u2018citizens\u2019 and \u2018journalists\u2019 become blurred. The emphasis appears to be on change more than continuity, and on difference more than similarity. Although the hybrid media approach is appreciated by numerous contemporary media scholars, hybridity in media often remains an all-encompassing concept and few attempts have been made to measure it. This article assesses the level of hybridity by investigating journalists\u2019 uses of sources. It considers mainly journalists\u2019 use of sources by the medium for which they work (from newspapers to web or radio) and the kinds of news that they produce (hard or soft news, business/finance, tech/science). The contemporary media systems present hybrid logics and features that imply an increasingly interdependence among actors, media and communication formats. The hybrid media system approach underlines that legacy news media and non-elite media actors construct flows of news through different media technologies and according to complex temporal structures. A media environment arises in which traditional distinctions between concepts like \u2018online\u2019 and \u2018offline\u2019, \u2018producer\u2019 and \u2018audience\u2019, \u2018citizens\u2019 and \u2018journalists\u2019 become blurred. The emphasis appears to be on change more than continuity, and on difference more than similarity. Although the hybrid media approach is appreciated by numerous contemporary media scholars, hybridity in media often remains an all-encompassing concept and few attempts have been made to measure it. This article assesses the level of hybridity by investigating journalists\u2019 uses of sources. It considers mainly journalists\u2019 use of sources by the medium for which they work (from newspapers to web or radio) and the kinds of news that they produce (hard or soft news, business/finance, tech/science). The assumption is, therefore, that, within a homogeneously hybridized media system, journalists use the same sources regardless of the medium for which they work and the topics with which they deal. This objective is pursued by analysing the data collected via a survey conducted by means of structured interviews with a sample of 1424 Italian journalists between October and November 2016. The findings show that the analytical distinction among platforms for which journalists work still matters in terms of sources. Except for the use of Facebook and Google, journalists have still very defined paths to collect sources according to the medium they work for. The article has implications also for the literature on journalists\u2019 authority and expertis

    Internazionalizzare gli studi sul giornalismo di mafia

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    Organized crime and mafia have been studying by methods that are increasingly rigorous. Within the subfield of mafia journalism, a more solid approach appears to be less evident, despite many contributions on the subject. This article argues the need for a deeper link with the so-called journalism studies, international studies dealing with journalism. The article states that in this context the studies on mafia journalism can find useful theoretical perspectives and methodologies. At the same time, the study of journalism inherent organized crime is a fundamental subfield of the more general studies on journalism

    From contents to comments : Social TV and perceived pluralism in political talk shows

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    Going beyond source and content pluralism, we propose a two-dimensional audiencebased measure of perceived pluralism by exploiting the practice of \u201csocial TV\u201d. For this purpose, 135,228 tweets related to 30 episodes of prime time political talk shows broadcast in Italy in 2014 have been analyzed through supervised sentiment analysis. The findings suggest that the two main TV networks compete by addressing generalist audiences. The public television offers a plural set of talk shows but ignores the antipolitical audience. The ideological background of the anchorman shapes the audience\u2019s perception, while the gender of the guests does not seem to matter

    Reduced transcription of TCOF1 in adult cells of Treacher Collins syndrome patients

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    <p>Abstract</p> <p>Background</p> <p>Treacher Collins syndrome (TCS) is an autosomal dominant craniofacial disorder caused by frameshift deletions or duplications in the <it>TCOF1 </it>gene. These mutations cause premature termination codons, which are predicted to lead to mRNA degradation by nonsense mediated mRNA decay (NMD). Haploinsufficiency of the gene product (treacle) during embryonic development is the proposed molecular mechanism underlying TCS. However, it is still unknown if <it>TCOF1 </it>expression levels are decreased in post-embryonic human cells.</p> <p>Methods</p> <p>We have estimated <it>TCOF1 </it>transcript levels through real time PCR in mRNA obtained from leucocytes and mesenchymal cells of TCS patients (n = 23) and controls (n = 18). Mutational screening and analysis of NMD were performed by direct sequencing of gDNA and cDNA, respectively.</p> <p>Results</p> <p>All the 23 patients had typical clinical features of the syndrome and pathogenic mutations were detected in 19 of them. We demonstrated that the expression level of <it>TCOF1 </it>is 18-31% lower in patients than in controls (<it>p < 0.05</it>), even if we exclude the patients in whom we did not detect the pathogenic mutation. We also observed that the mutant allele is usually less abundant than the wild type one in mesenchymal cells.</p> <p>Conclusions</p> <p>This is the first study to report decreased expression levels of <it>TCOF1 </it>in TCS adult human cells, but it is still unknown if this finding is associated to any phenotype in adulthood. In addition, as we demonstrated that alleles harboring the pathogenic mutations have lower expression, we herein corroborate the current hypothesis of NMD of the mutant transcript as the explanation for diminished levels of <it>TCOF1 </it>expression. Further, considering that <it>TCOF1 </it>deficiency in adult cells could be associated to pathologic clinical findings, it will be important to verify if TCS patients have an impairment in adult stem cell properties, as this can reduce the efficiency of plastic surgery results during rehabilitation of these patients.</p

    Genome Analysis of the Domestic Dog (Korean Jindo) by Massively Parallel Sequencing

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    Although pioneering sequencing projects have shed light on the boxer and poodle genomes, a number of challenges need to be met before the sequencing and annotation of the dog genome can be considered complete. Here, we present the DNA sequence of the Jindo dog genome, sequenced to 45-fold average coverage using Illumina massively parallel sequencing technology. A comparison of the sequence to the reference boxer genome led to the identification of 4 675 437 single nucleotide polymorphisms (SNPs, including 3 346 058 novel SNPs), 71 642 indels and 8131 structural variations. Of these, 339 non-synonymous SNPs and 3 indels are located within coding sequences (CDS). In particular, 3 non-synonymous SNPs and a 26-bp deletion occur in the TCOF1 locus, implying that the difference observed in cranial facial morphology between Jindo and boxer dogs might be influenced by those variations. Through the annotation of the Jindo olfactory receptor gene family, we found 2 unique olfactory receptor genes and 236 olfactory receptor genes harbouring non-synonymous homozygous SNPs that are likely to affect smelling capability. In addition, we determined the DNA sequence of the Jindo dog mitochondrial genome and identified Jindo dog-specific mtDNA genotypes. This Jindo genome data upgrade our understanding of dog genomic architecture and will be a very valuable resource for investigating not only dog genetics and genomics but also human and dog disease genetics and comparative genomics

    Impact of complex NOTCH1 mutations on survival in paediatric T-cell leukaemia

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    <p>Abstract</p> <p>Background</p> <p>Molecular alterations occur frequently in T-ALL and the potential impact of those abnormalities on outcome is still controversial. The current study aimed to test whether <it>NOTCH1 </it>mutations and additional molecular abnormalities would impact T-ALL outcome in a series of 138 T-ALL paediatric cases.</p> <p>Methods</p> <p>T-ALL subtypes, status of <it>SIL-TAL1 </it>fusion, ectopic expression of <it>TLX3</it>, and mutations in <it>FBXW7</it>, <it>KRAS</it>, <it>PTEN </it>and <it>NOTCH1 </it>were assessed as overall survival (OS) and event-free survival (EFS) prognostic factors. OS and EFS were determined using the Kaplan-Meier method and compared using the log-rank test.</p> <p>Results</p> <p>The frequencies of mutations were 43.5% for <it>NOTCH1</it>, while <it>FBXW7</it>, <it>KRAS </it>and <it>PTEN </it>exhibited frequencies of 19.1%, 9.5% and 9.4%, respectively. In 78.3% of cases, the coexistence of <it>NOTCH1 </it>mutations and other molecular alterations was observed. In multivariate analysis no statistical association was revealed between <it>NOTCH1 </it>mutations and any other variable analyzed. The mean length of the follow-up was 68.4 months and the OS was 50.7%. <it>SIL-TAL1 </it>was identified as an adverse prognostic factor. <it>NOTCH1 </it>mutation status was not associated with outcome, while the presence of <it>NOTCH1 </it>complex mutations (indels) were associated with a longer overall survival (<it>p </it>= 0.031) than point mutations.</p> <p>Conclusion</p> <p><it>NOTCH1 </it>mutations alone or in combination with <it>FBXW7 </it>did not impact T-ALL prognosis. Nevertheless, complex <it>NOTCH1 </it>mutations appear to have a positive impact on OS and the <it>SIL-TAL1 </it>fusion was validated as a negative prognostic marker in our series of T-ALL.</p

    The history of leishmaniasis

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    In this review article the history of leishmaniasis is discussed regarding the origin of the genus Leishmania in the Mesozoic era and its subsequent geographical distribution, initial evidence of the disease in ancient times, first accounts of the infection in the Middle Ages, and the discovery of Leishmania parasites as causative agents of leishmaniasis in modern times. With respect to the origin and dispersal of Leishmania parasites, the three currently debated hypotheses (Palaearctic, Neotropical and supercontinental origin, respectively) are presented. Ancient documents and paleoparasitological data indicate that leishmaniasis was already widespread in antiquity. Identification of Leishmania parasites as etiological agents and sand flies as the transmission vectors of leishmaniasis started at the beginning of the 20th century and the discovery of new Leishmania and sand fly species continued well into the 21st century. Lately, the Syrian civil war and refugee crises have shown that leishmaniasis epidemics can happen any time in conflict areas and neighbouring regions where the disease was previously endemic
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